Composite

Part:BBa_K5036027:Design

Designed by: Emad hamdy Matter   Group: iGEM24_AFCM-Egypt   (2024-09-21)


dCas9(C)_NLS-Syn-VEGFR-1(VEGF-R1, C-TEV, NLS, TCS(Q,G),HA,dCas9(C), VP64, GAL4, GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2173
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 6308
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3973
    Illegal NgoMIV site found at 4046
    Illegal NgoMIV site found at 4531
    Illegal NgoMIV site found at 5440
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6227
    Illegal BsaI.rc site found at 7175
    Illegal SapI.rc site found at 3268


Design Notes

NO


Source

ADDGENE

References